Q_T-Resummation for Polarized Semi-Inclusive Deep Inelastic Scattering

We study the transverse-momentum distribution of hadrons produced in semi-inclusive deep-inelastic scattering. We consider cross sections for various combinations of the polarizations of the initial lepton and nucleon or the produced hadron, for which we perform the resummation of large double-logarithmic perturbative corrections arising at small transverse momentum. We present phenomenological results for the process $ep\to e\pi X$ for the typical kinematics in the COMPASS experiment. We discuss the impact of the perturbative resummation and of estimated non-perturbative contributions on the corresponding cross sections and their spin asymmetry.Comment: Talk presented at DIS2006. 4 pages, 3 figure

Toxicity and Molecular Identification of Green Toadfish Lagocephalus lunaris Collected from Kyushu Coast, Japan

Green toadfish Lagocephalus lunaris inhabits tropical and subtropical seas and contains high tetrodotoxin (TTX) levels in the muscle as well as liver and gonad. In 2008 to 2009, food poisoning due to ingesting L. lunais occurred in Western Japan. Five specimens of green toadfish caught in Kyushu coast, Japan, were analyzed for toxicity, toxins, and species identification. All five specimens were toxic by bioassay. Comparing the maximum toxicity in tissues, ovary contained the most toxin (1810 mouse unit [MU]/g), followed by liver (341 MU/g), muscle (135 MU/g), skin (79 MU/g), and intestine (72 MU/g). Liquid chromatography/mass spectrometry analysis revealed that TTX was the major toxin. Nucleotide sequence analysis of the 16S rRNA gene fragment of muscle mitochondrial DNA indicated that partial sequences of PCR products of four specimens were identical with that of L. lunaris. The sequence of one specimen was indistinguishable from that of the brown-backed toadfish Lagocephalus wheeleri, a nontoxic species

ホタテガイの中腸腺由来金属結合タンパク質の分離

Marine bivalves accumulate high concentrations of potentially toxic heavy metals in their tissues. We report here our investigation of the accumulation patterns of cadmium (Cd), copper (Cu), and lead (Pb) in tissues of the Japanese scallop Mizuhopecten yessoensis after exposure to artificial seawater containing Cd, Cu, or Pb. Our results revealed that accumulation of these heavy metals by this scallop was associated with certain intracellular metal-binding proteins (MBPs). Cd was accumulated at higher concentrations than Cu and Pb, and most of the accumulated metals were detected in the digestive gland. MBPs from the digestive gland were purified and subjected to a preliminary characterization. Three proteins with molecular masses of approximately 28, 37, and 42 kDa, respectively, were isolated by gel-filtration and anion-exchange column chromatography. Characterization of the partial amino acid sequences of these proteins show high sequence similarity to MBPs and ion-transporters. Metalloprotein profiles in the digestive gland indicated that some proteins were upregulated after metal exposure, suggesting that these proteins are involved in mechanisms of metal accumulation and detoxification in M. yessoensis.ホタテガイをカドミウム，銅または鉛を含む人工海水に暴露した後，金属の蓄積能および金属結合タンパク質の存在を調べた。いずれの金属も中腸腺に顕著に蓄積され，その蓄積量はカドミウム，銅，鉛の順に高かった。中腸腺から分子量約 28, 37 および 42 kDa の金属結合タンパク質が精製され，これらのアミノ酸部分配列解析により，Coccidioides immitis の calcium-binding protein または Pleurocapsa sp. の ion-transporter 類似タンパク質と高い相同性を示すことを明らかにした。これらのタンパク質はホタテガイの金属蓄積または解毒メカニズムに関与していることが示唆された

Non-B hepatocellular carcinoma: influence of age, sex, alcohol, family clustering, blood transfusion and chronic liver disease.

In 144 cases of hepatocellular carcinoma (HCC), 166 cases of cirrhosis without HCC and 142 cases of chronic hepatitis, we examined HBsAg, anti-HBs and anti-HBc in sera and compared the following factors between hepatitis B virus marker-negative and -positive patients: age, sex, alcohol consumption, family clustering of liver diseases, and histories of blood transfusion and post-transfusion hepatitis. Results of this study demonstrated several distinct differences in clinical backgrounds between non-B (negative for HBsAg, anti-HBs and anti-HBc) and B (positive for HBsAg) patients with HCC. Non-B patients were significantly older, had a lower frequency of familial tendencies for liver diseases, and more frequently had cancers other than HCC in their families. Some of these differences were also observed between non-B and B patients with cirrhosis and chronic hepatitis. Among patients with chronic hepatitis, the non-B patients had received blood transfusion or had post-transfusion hepatitis more frequently than the B patients. However, this difference was not apparent in patients with liver cirrhosis or HCC, suggesting that progression of non-A, non-B post-transfusion hepatitis to cirrhosis and HCC may not be as frequent as progression to chronic hepatitis.</p

Hepatitis B virus associated particles in the bile canaliculus.

The liver biopsy specimen from a patient with hepatitis B surface antigen was pbserved by electron microscopy. Dane particles, uncoated core particles and tubular forms were demonstrated in hepatocytes. Dane particles and tubular forms, approximately 25nm in diameter, were also found in the bile canaliculi. These findings suggest that hepatitis B virus and associated particles are released from hepatocytes into the bile duct

Reactivity of IgE in fish-allergic patients to fish muscle collagen

ABSTRACTBackground: In addition to parvalbumin, the well- known major allergen in fish, collagen was recently identified as a new allergen in the muscle of bigeye tuna and in the skin of several species of fish. The aim of the present study was to evaluate fish muscle colla- gens for their reactivity with IgE in fish-allergic patients and antigenic cross-reactivity.Methods: Collagen was purified from the white muscle of five species of fish (Japanese eel, alfonsin, mackerel, skipjack and bigeye tuna) by acid extraction and salt precipitation, whereas parvalbumin was purified from bigeye tuna by gel filtration and reverse- phase HPLC. The IgE reactivities to collagen and parvalbumin were examined by ELISA, whereas antigenic cross-reactivity among fish muscle collagens was investigated by ELISA inhibition experiments.Results: When 15 sera from fish-allergic patients were subjected to ELISA using bigeye tuna collagen and parvalbumin, 10 sera reacted only to parvalbumin, two reacted only to collagen, two reacted to both collagen and parvalbumin and one reacted to neither collagen nor parvalbumin. The sera containing specific IgE to bigeye tuna collagen also reacted to collagens from the other four species of fish. In the ELISA inhibition experiments, bigeye tuna collagen inhibited the binding of IgE not only to bigeye tuna collagen, but also to that from the other four species of fish, suggesting cross-reactivity among the collagens from five species of fish.Conclusions: These results demonstrate that some Japanese fish-allergic patients have specific IgE to fish muscle collagen and that fish muscle collagen is a cross-reactive allergen among various species of fish

Electron microscopic observation of hepatitis B virus budding from hepatocytes into bile canaliculi.

In electron microscopic observation of a liver biopsy obtained from a hepatitis B surface antigen-positive patient, noncoated core particles were occasionally seen budding into the hepatocytic cisterni and many Dane particles were found in the pericanalicular vesicles of hepatocytes. Noncoated core particles were also localized in clusters within the bleb of microvilli. There were some core particles being protruded from microvilli into the lumen of bile canaliculi by budding. These findings suggest hepatitis B virus being released from the hepatocyte to the bile canaliculi by two different modes; through vesicle by reversed phagocytosis and from the microvilli by budding.</p

細菌の接種によって誘導されるアコヤガイ中の抗菌性タンパク質の探索

The aim of this research was to characterize immune-related antibacterial substances from pearl oyster Pinctada fucata induced by bacterial invasion. Bacteria inoculation was performed by injecting 0.1 ml of 1.0 × 1012 colony-forming units/ml Vibrio parahaemolyticus into adductor muscle. Acidic extracts were prepared with 0.1% trifluoroacetic acid from different tissues after 8 h of injection, and antibacterial activity against V. parahaemolyticus was determined via the microdilution broth method. The acidic extracts from gills of inoculated oysters (AEg) showed stronger antibacterial activity than those from non-inoculated ones. Based on this result, antibacterial proteins were purified from AEg via two-step gel filtration chromatography, followed by high-performance liquid chromatography using a TSkgel G3000 column. Protein components were analyzed by both sodium dodecyl sulfate and native polyacrylamide gel electrophoresis. As a result, two antibacterial proteins, APg-1 (with a molecular mass of approximately 210 kDa) and APg-2 (of approximately 30 kDa), were obtained from AEg. Matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis and partial amino acid sequences revealed that these proteins might be novel antibacterial proteins. These results indicate that antibacterial proteins are potentially upregulated in the gill of pearl oysters or released therefrom for defense against bacterial invasion.本研究で対象とするアコヤガイは，日本において重要な養殖真珠の母貝として用いられる二枚貝である。腸炎ビブリオをアコヤガイ閉殻筋に直接接種したところ，鰓から得られた酸抽出物に，非接種の対照よりも強い抗菌活性を示す成分が存在することを見出した。酸抽出物はグラム陽性菌および陰性菌に抗菌活性を示し，とくにビブリオ属に強く作用した。鰓より2種の抗菌タンパク質APg-1（分子量約210 kDa）およびAPg-2（分子量約30 kDa）を分離し，これらはMALDI-TOF MS分析により，新規の抗菌タンパク質である可能性が推察された